![]() ![]() We address the misleading way DLS size distributions are often presented, that is, as a logarithmically scaled histogram of measured relative quantities. #DYNAMIC LIGHT SCATTERING SIZE RANGE SOFTWARE#Here, we critically discuss the application of DLS for nanoparticle characterization and provide much-needed clarification for ambiguities in the mean-value practice of commercial DLS software and documentary standards. Please follow the link to our light scattering theory page to learn more about dynamic and static light scattering theory.Dynamic light scattering (DLS) is an essential technique for nanoparticle size analysis and has been employed extensively for decades, but despite its long history and popularity, the choice of weighting and mean of the size distribution often appears to be picked ad hoc to bring the results into agreement with other methods and expectations by any means necessary. Owing to the technique’s high sensitivity towards large species, even small impurities caused by aggregation can be detected Owing to its large measurement range (0.5 nm – 1 µm) DLS closes the gap between the range of small soluble aggregates that are detected via size exclusion chromatography (SEC) and particles analyzed by methods such as light obscuration (LO) or micro flow imaging (MFI). ZentriForce Pharma offers DLS services under BSL 1 and 2.ĭynamic light scattering is known as a fast and sensitive screening method for aggregate / particle detection and therefore widely used within biopharmaceutical development. We routinely conduct biosimilarity and gene therapy studies and are furthermore experienced in the development of dynamic light scattering (DLS) methods for a large variety of samples. Zentriforce Pharma is a contract research organization that specializes in analytical services with a focus on hydrodynamic methods.
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